A01 - Defining insulin targets in control of feeding-associated reward behaviour

The lateral hypothalamus (LH) serves as an integrator in the homeostatic and hedonic regulation of feeding. As both of these mechanisms are regulated by insulin action in the CNS, we aim to specifically define the role of insulin signalling in the LH in control of food reward in vivo. We will define insulin-stimulated signals and insulin-evoked alterations in neuroactivation of these cells. Moreover, we aim to disrupt insulin signalling specifically in MCH- and orexin-expressing neurons in the LH of mice and assess the effects on energy homeostasis and glucose metabolism.